A real-time qualitative PCR test that is based on the combination of allele-specific priming, asymmetric PCR, and melting curve analysis for the detection of PIK3CA mutations (E542K, E545K, E545Q, H1047R). The kit can be used on DNA samples extracted from FFPE tumor tissues, circulating tumor cells (CTCs) and plasma of breast cancer patients.
The Oncolipsy PIK3CA Kit (CE-IVD) offers:
- High sensitivity and specificity
- Simple set-up and interpretation of results through melting curve analysis
- Ready-to-use and stable master-mixes
- Quality control testing
Procedure of Analysis
Oncolipsy PIK3CA Kit - A simple procedure in three steps.
Instructions for Use (IFU)
The Oncolipsy PIK3CA kit includes sufficient reagents to analyze 24 samples including PCR positive and negative controls. Each Oncolipsy PIK3CA kit contains 14 vials with different color caps (red, green, blue, orange, white, yellow, light blue, and colourless) including PCR buffer master-mixes, primers/probe master-mixes, dye reagent, positive controls for each biomarker, and nuclease-free water along with a leaflet.
Published paper
Markou A. et al. “PIK3CA mutational status in circulating tumor cells can change during disease recurrence or progression in patients with breast cancer” Clin Cancer Res. 2014 Nov 15;20(22):5823-34.